Description
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BSA-Ab-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated BSA-Ab-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled BSA-Ab-IgG and unlabeled BSA-Ab-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of BSA-Ab-IgG in the sample. The color development is stopped and the intensity of the color is measured